Phospholipid capture combined with non-linear chromatographic correction for improved serum metabolite profiling |
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Authors: | Elizabeth J Want Colin A Smith Chuan Qin K C Van Horne Gary Siuzdak |
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Institution: | (1) The Center for Mass Spectrometry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA;(2) Tandem Labs-Salt Lake City, 1121 East 3900 South, Salt Lake City, UT 84124, USA |
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Abstract: | Serum analysis with LC/MS can yield thousands of potential metabolites. However, in metabolomics, biomarkers of interest will often be of low abundance, and ionization suppression from high abundance endogenous metabolites such as phospholipids may prevent the detection of these metabolites. Here a cerium-modified column and methyl-tert-butyl-ether (MTBE) liquid–liquid extraction were employed to remove phospholipids from serum in order to obtain a more comprehensive metabolite profile. XCMS, an in-house developed data analysis software platform, showed that the intensity of existing endogenous metabolites increased, and that new metabolites were observed. This application of phospholipid capture in combination with XCMS non-linear data processing has enormous potential in metabolite profiling, for biomarker detection and quantitation. |
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Keywords: | phospholipid metabolite profiling liquid chromatography mass spectrometry serum |
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