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Identification of RAPD markers linked to a Phytophthora fragariae resistance gene (Rpf1) in the cultivated strawberry |
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| Authors: | K. M. Haymes B. Henken T. M. Davis W. E. van de Weg |
| Affiliation: | (1) Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Vegetable and Fruit Crops, PO Box 16, 6700 AA Wageningen, The Netherlands, NL;(2) University of New Hampshire, Department of Plant Biology, Rudman Hall, Durham, NH 03824, USA, US |
| Abstract: | Bulked segregant analysis (BSA) was used to identify seven random amplified polymorphic DNA (RAPD) markers linked to the Rpf 1 gene. Rpf 1 confers resistance to Phytophthora fragariae var. fragariae, the causal agent of red stele root rot in Fragaria spp. The bulked DNAs represented subsets of a F1 population obtained from the cross Md683×Senga Sengana which consisted of 60 plants and segregated in a 1:1 ratio for resistance or susceptibility to race 2.3.4 isolate NS2 of P. fragariae. Seven markers were shown to be linked to Rpf 1 and were generated from four primers; five of these markers were in coupling phase and two in repulsion phase with respect to the gene. A linkage map of this resistance gene region was generated using JoinMap 2.0TM. The manner in which Rpf 1 and the linked markers co-segregated indicated that they are inherited in a disomic fashion. These markers could enable gene pyramiding and marker-assisted selection of resistance genes in strawberry breeding programmes. Received: 26 August 1996 / Accepted: 20 December 1996 |
| Keywords: | Red stele Linkage-analysis Fragaria×≧ ≤ ananassa Bulked-segregant-analysis (BSA) Resistance-gene mapping |
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