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Genetics and mapping of new isozyme loci in Vicia faba L using trisomics
Authors:A. M. Torres  Z. Satovic  J. Canovas  S. Cobos  J. I. Cubero
Affiliation:(1) C.I.D.A., Departamento de Mejora y Agronomía, Aptdo 4240, 14080 Córdoba, Spain;(2) Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, 41000 Zagreb, Croatia;(3) Departamento de Genética, E.T.S.I.A.M., Aptdo 3048, 14080 Córdoba, Spain
Abstract:Polymorphism in ten enzyme systems (ACO, ACP, AAT, EST, FK, ME, NAG, PRX, 6PGD, and SOD) in Vicia faba L. was analyzed, revealing 13 loci, six of which have not been reported before. Inheritance, genetics, possible location, and linkage analysis were studied in 13 different F2 populations trisomic for four of the six chromosomes (nos. 3, 4, 5, and 6) of the species. Each of these loci exhibited typical Mendelian inheritance except for those involved in the trisomic chromosome. Five loci have been assigned to a specific chromosome: Est-2 to chromosome 3, Fk-2 to chromosome 4, Prx-1 to chromosome 5, and Sod-1 and Pgd-p to chromosome 6. Nag-1 and Pgd-c displayed a linkage of 22.8 cM indicating a clear homology with chromosome 5 of garden pea on which both markers are syntenic.
Keywords:Vicia faba  Isozyme  Trisomic  Mapping
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