d-Psicose production from d-fructose using an isolated strain, Sinorhizobium sp. |
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| Authors: | Deok-Kun Oh Nam-Hee Kim Hye-Jung Kim Chang-Su Park Seon Won Kim Minsu Ko Bueng Wan Park Min Ho Jung Ki-Hong Yoon |
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| Affiliation: | (1) Department of Bioscience and Biotechnology, Konkuk University, Seoul , 143-701, Korea;(2) Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Korea;(3) Institute of Molecular Genetics, Solgent Co., Ltd., Daejeon, 305-348, Korea;(4) School of Food Science and Biotechnology, Woosong University, 17-2 Jayang-dong, Dong-gu, Daejeon, 300-718, Korea |
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| Abstract: | ![]()
Sinorhizobium sp., which can convert d-fructose into d-psicose, was isolated from soil. The optimal pH, temperature, and cell concentration for d-psicose production with the isolated strain were 8.5, 40°C, and 60 mg/ml, respectively. The toluene-treated cells showed 2.5- and 4.8-fold increases in the d-psicose concentration and productivity compared with untreated washed cells. Under the optimal conditions, the toluene-treated cells produced 37 g d-psicose/l from 70% (w/v) (3.9 M) d-fructose after 15 h. |
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| Keywords: | Biotransformation d-Fructose Isolated strain d-Psicose Sinorhizobium sp. |
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