Introduction of foreign DNA into Chlorella saccharophila by electroporation |
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| Authors: | Masato Maruyama Irena Horáková Hiroyuki Honda Xin-hui Xing Naohiro Shiragami Hajime Unno |
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| Affiliation: | (1) Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, 227 Yokohama, Japan;(2) Oral Care Research Laboratory, Lion Limited Corporation, Japan;(3) U'stav Plicni'ch Nemoci', Praha, The Czech Republic;(4) Dept of Biotechnology, Faculty of Engineering, Nagoya University, Nagoya, Japan |
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| Abstract: | Summary Plasmid, pBI221, was introduced into protoplasts of Chlorella saccharophila c-211-1a prepared from the cells in the stationary phase by electroporation. Transient expression of the introduced plasmid was observed under a field strength of between 600 and 900 V/cm, and a pulse duration of around 400 ms, where high membrane permeability to 70-kDa FITC-dextran was ascertained. |
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