海藻酸钠/壳聚糖微胶囊固定化大肠杆菌的研究

本文以大肠杆菌ＤＨ5α为模型体系 ,探索了大肠杆菌ＤＨ5α用海藻酸钠 壳聚糖 (ＡＣＡ)微胶囊培养的可行性 ,并观察了微囊化大肠杆菌ＤＨ5α细胞生长与物料渗透性能 ,通过将ＡＣＡ微胶囊移植到实验组小鼠体内 ,考察了ＡＣＡ微胶囊作为口服药物载体的可能性。1　材料和方法1.1　材料壳聚糖 ,本实验室改性所得 ;海藻酸钠 ,ＫｅｌｃｏＤｉｖｏｆＭｅｒ ｃｋＣｏ .Ｉｎｃ .ＵＳＡ ;其它试剂均为国产分析纯。大肠杆菌ＤＨ5α ,长春生物制品所 ;ＬＢ培养基 ,华美生物制品公司提供。昆明系小白鼠 18～ 2 0ｇ ,解放军大连高等医学专科学校实验动物中…

Study of Alginate/Chitosan Microcapsules for Immobilization of Escherichia coli DH5α

Objective proteins synthesized from genetically recombined Escherichia coli strain (E. coli) have been successfully produced by microbe fermentation, but complicated separation and purification steps always make against the maintenance of activities as well as increase the cost. Aiming at simplifying the process, an idea of administrating directly the microencapsulated genetically recombined E. coli is proposed. In this paper, study on culture of E. coli DH5 alpha immobilized in alginate/chitosan (ACA) microcapsule is presented. It was found that E. coli DH5 alpha grew well in the microcapsule with stable growth period longer than that of suspension culture, and cell aggregation phenomenon was observed. In vivo experiments showed that ACA microcapsules with E. coli DH5 alpha stayed over 48 h in mouse intestine, and the morphology of microcapsules was kept intact. These preliminary results have demonstrated that administration of microencapsulated E. coli DH5 alpha is safe, which laied the foundation for microencapsulated genetically recombined E. coli as carriers of gene engineering drugs.