| 大百合小孢子发生和雄配子体发育研究 |
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| 引用本文： | 关文灵,李世峰,雷丽萍,李叶芳,陈贤.大百合小孢子发生和雄配子体发育研究[J].亚热带植物科学,2011(4):12-15. |
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| 作者姓名： | 关文灵 李世峰 雷丽萍 李叶芳 陈贤 |
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| 作者单位： | 云南农业大学园林园艺学院;云南省农业科学院花卉研究所;昆明市翠湖公园; |
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| 基金项目： | 国家教育部“春晖”计划项目(Z2005-2-65007); 云南省自然科学基金项目(2007C0059M)资助 |
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| 摘 要： | 用常规石蜡切片技术和压片法对大百合小孢子发生和雄配子体发育进行观察。结果表明:花药4室,花药壁由表皮、药室内壁、中层和腺质绒毡层组成,花药壁发育方式为单子叶型,药室内壁部分细胞发育后期发生纤维状加厚。小孢子母细胞减数分裂过程的胞质分裂为连续型,四分体多数为左右对称型,偶有四面体型。成熟花粉为2细胞型,具1个萌发沟。经TTC法检验,成熟花粉生活力为86.3%。从小孢子的发生及雄配子体发育的整个过程看,未见异常现象,能形成大量正常的成熟花粉。
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| 关 键 词： | 大百合 小孢子发生 雄配子体 花粉生活力 |
Study on Microsporogenesis and Development of Male Gametophyte in Cardiocrinum giganteum |
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| GUAN Wen-ling,LI Shi-feng,LEI Li-ping,LI Ye-fang,CHEN Xian.Study on Microsporogenesis and Development of Male Gametophyte in Cardiocrinum giganteum[J].Subtropical Plant Science,2011(4):12-15. |
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| Authors: | GUAN Wen-ling LI Shi-feng LEI Li-ping LI Ye-fang CHEN Xian |
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| Institution: | GUAN Wen-ling1,LI Shi-feng2,LEI Li-ping3,LI Ye-fang1,CHEN Xian1 (1.College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,Yunnan China,2.Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,3.Green Lake Park,Yunnan China) |
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| Abstract: | The microsporogenesis and development of male gametophyte in Cardiocrinum giganteum were observed by the means of conventional paraffin sections and norm slice-making method.The results show that the anther has four chambers and the structure of anther wall is the monocotyledonous type,which is composed of epidermis,endothecium,middle layer and glandular tapetum,some cells of the endothecium become fibrously thickened at anther dehiscence.The cytokinesis of microspore mother cell meiosis is successive,and t... |
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| Keywords: | Cardiocrinum giganteum microsporogenesis male gametophyte vitality rate of pollen grain |
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