Development of Pollen Embryoids in Anther Cultures of Datura innoxia: II. EFFECTS OF GROWTH HORMONES

In anther cultures of Datura innoxia the addition of growthhormones, such as auxins, gibberellins, and cytokinins, to theculture medium enhanced the production of pollen embryoids.Cytokinins appeared to be the most effective and, among thefour cytokinins tested, zeatin and kinetin gave the best results.Generally speaking, combinations of hormones did not improvethe response over that of an individual hormone. The numberof embryoids per anther varied in the same medium and did notstrictly correlate with the percentage of responding anthers.