Dialysable leucocyte extract (DLE) reduces lipopolysaccharide- induced tumour necrosis factor secretion in human leucocytes |
| |
Authors: | Dr Miriam Ojeda Celia B Fernandez Ortega Manuel de J Araña Rosaínz |
| |
Institution: | (1) Department of Cell Biology, Center for Biological Research, P.O. Box 6996, Havana, Cuba |
| |
Abstract: | Dialysable leucocyte extract (DLE), obtained from lysed leucocytes, provide clinical effectiveness in a broad spectrum of
diseases. Tumour necrosis factor (TNF) is raised in AIDS patients leading to an increase in human immunodeficiency virus (HIV)
replication in vitro 1,2], whereas progression to AIDS in asymptomatic HIV infected individuals is retarded under treatment
with DLE. In the present study we tested the DLE effect in vitro on both TNF biological activity (cytotoxicity) in L929 cells
and its induction by lipopolysaccharide (LPS) in human monocytes as well as in whole blood from healthy donors. When monocytic
cells were simultaneously exposed to LPS and DLE during a period of 5 1/2 hours, the induction of TNF was strongly diminished.
The same inhibitory effect of DLE on TNF induction was observed when LPS was added to the culture medium prior to DLE. No
significant effect of DLE on TNF-mediated cytotoxicity, even in the presence of the highest concentrations of DLE tested,
was detected. DLE treatment of whole human blood regulates responses to LPS: simultaneous in vitro exposure to endotoxin provokes
a remarkable decrease (4- and 1.6-fold) of TNF release. In pre-incubation experiments, TNF production was largely reduced
or completed abrogated. These results could, in part, explain the in vivo observed effect, when under treatment with this
extract, the progression to AIDS of HIV-infected individuals was retarded. The results suggest that ‘natural’ substances like
DLE may be important immunomodulators in inflammatory diseases. |
| |
Keywords: | Dialysable leucocyte extract lipopolysaccharide transfer factor tumour necrosis factor |
本文献已被 SpringerLink 等数据库收录! |
|