| 拟细羽束梗孢发酵菌丝体中自由基清除活性成分分析 |
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| 引用本文： | 李康乐,王小董,张德龙,陆瑞利,胡丰林.拟细羽束梗孢发酵菌丝体中自由基清除活性成分分析[J].微生物学通报,2011,38(11):1740-1746. |
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| 作者姓名： | 李康乐 王小董 张德龙 陆瑞利 胡丰林 |
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| 作者单位： | 1. 安徽农业大学微生物防治省重点实验室 安徽 合肥 230036
2. 安徽农业大学应用化学系 安徽 合肥 230036 |
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| 基金项目： | 国家自然科学基金项目(No. 30871676); 安徽省自然科学基金项目(No. 11040606M69) |
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| 摘 要： | 用二苯基苦基苯肼(DPPH)自由基法,首次对拟细羽束梗孢(Isaria gracilioides RCEF3 279)菌丝体的不同溶剂提取物进行了自由基清除活性的定性定量分析,发现其甲醇提取物具有较强的清除自由基活性,当菌丝浓度为10 g/L时,其甲醇提取物的自由基清除率达到了92.4％±0.3％.DPPH自显影-薄层...
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| 关 键 词： | 拟细羽束梗孢 清除自由基 活性测定 液质联用 二苯基苦基苯肼(DPPH) |
Analysis of radical scavenging active constituents in the mycelia of Isaria gracilioides |
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| LI Kang-Le,WANG Xiao-Dong,ZHANG De-Long,LU Rui-Li and HU Feng-Lin.Analysis of radical scavenging active constituents in the mycelia of Isaria gracilioides[J].Microbiology,2011,38(11):1740-1746. |
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| Authors: | LI Kang-Le WANG Xiao-Dong ZHANG De-Long LU Rui-Li and HU Feng-Lin |
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| Institution: | 1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, Anhui 230036, China;1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, Anhui 230036, China;1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, Anhui 230036, China;2. Department of Applied Chemistry, Anhui Agricultural University, Hefei, Anhui 230036, China;1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, Anhui 230036, China |
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| Abstract: | A DPPH-microplate assay was employed for quantitative analysis of the free radical scavenging activity of the extracts extracted with different solvents from mycelia of Isaria gracilioides. The results showed that the methanol extract had the strongest free radical scavenging activity. At the concentration of 10 mg mycelium per milliliter methanol, the radical scavenging rate of the extract reached 92.4%±0.3%. The results of DPPH-TLC assays showed that the methanol extract contained four main free radical scavenging components. The combined LC-DAD-HRMS and DPPH assay revealed that the molecular formulas of the four free radical scavengers were possibly C15H16NO3 (1), C32H40N2O18 (2), C30H55N5O5 (3) and C17H14N2O2 (4). Natural products database query showed that compounds 3 and 4 were possibly viscumamide and dehydrocyclopeptin, respectively, and compounds 1 and 2 were novel molecules. Viscumamide and dehydrocyclopeptin were detected from entomogenous fungi for the first time. |
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| Keywords: | Isaria gracilioides Ree radical scavenger LC-HRMS 1 1-Diphenyl-2-picryhydrazyl (DPPH) |
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