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Passive Ca overload in H9c2 cardiac myoblasts: Assessment of cellular damage and cytosolic Ca transients
Authors:Fernando Soler,Antonio Lax,M. Carmen Asensio,Domingo Pascual-Figal,Francisco Ferná  ndez-Belda
Affiliation:aDepartamento de Bioquímica y Biología Molecular A, Universidad de Murcia, Campus de Espinardo, 30071 Murcia, Spain;bDepartamento de Medicina Interna (Cardiología), Facultad de Medicina, Universidad de Murcia, Campus de El Palmar, 30120 Murcia, Spain
Abstract:Increase of resting Ca2+ levels and amplitude of vasopressin-induced Ca2+ transients were observed when cells in serum-free medium were exposed to 5 mM Ca2+ for 2 h. Small effect on cell viability was also observed. A rapid cytotoxic effect was developed in the presence of 10 mM Ca2+ and absence of serum. However, cells exposed to 10 mM Ca2+ in the presence of serum were protected from damage for at least 2 days. Resting Ca2+ levels and cytosolic Ca2+ transients in serum-containing medium with 10 mM Ca2+ displayed lower increases and a tendency to recover control values. When serum was absent, cells preincubated with 10 mM Ca2+ were more sensitive to thapsigargin-induced damage than cells preincubated with lower Ca2+. The sensitivity was similar when serum was present. Tolerance to high Ca2+ in the presence of serum was linked to potentiation of the mitochondrial Ca2+ entry to decrease the sarcoplasmic reticulum Ca2+ overload.
Keywords:Sarcoplasmic reticulum   Thapsigargin   Ca2+ overload   Ca2+ transient   Cell damage   H9c2 cell
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