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Plant regeneration from cultured hypocotyl explants of diploid and tetraploid Solanum khasianum clarke
Authors:Alka L Gunay  P S Rao
Institution:(1) Plant Morphogenesis and Tissue Culture Section, Bio-Organic Division, Bhabha Atomic Research Centre, 400085 Bombay, India;(2) Department of Biology, Wilson College, Bombay
Abstract:Plant regeneration through shoot bud formation was induced in cultured hypocotyl explants of diploid and tetraploid varieties of Solanum khasianum. Optimum regeneration of multiple shoot buds accompanied by rooting occurred on Murashige and Skoog's medium supplemented with IAA (1 mg/1) and kinetin or benzyladenine (1 mg/1). Plantlets obtained in vitro were successfully established in soil.Part of a thesis entitled ldquoMorphogenetic studies in tissue and organ cultures of certain Solanaceous plants: Capsicum, Lycopersicon and Solanumrdquo submitted by A.L. Gunay and approved for Master's degree in Botany by research of the University of Bombay.
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