Observations of Forsythia koreana methanol extract on mast cell-mediated allergic reactions in experimental models |
| |
Authors: | In-Young Choi Phil-Dong Moon Hyun-Na Koo Noh-Yil Myung Su-Jin Kim Ji-Hyun Lee Se-Hee Han Goo Moon Sung-Yum Seo Hyun-Jea Sung Rae-Kil Park Hyun-Ja Jeong Jae-Young Um Hyung-Min Kim Seung-Heon Hong |
| |
Institution: | (1) Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, South Korea;(2) College of Pharmacy, VestibuloCochlear Research Center of Wonkwang University, Iksan, Jeonbuk, 570-749, South Korea;(3) Graduate School of Alternative Medicine, Kyonggi University, Seoul, 120-702, South Korea;(4) College of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, South Korea;(5) Department of Biology, College of Natural Sciences, Kongju National University, Kongju, 314-701, South Korea |
| |
Abstract: | To explore effects of Forsythia koreana methanol extract (FKME) on mast cell-mediated allergic and inflammatory properties, the effect of FKME was evaluated on compound
48/80-induced systemic anaphylaxis, ear swelling, and anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-induced passive cutaneous
anaphylaxis (PCA). In addition, the effect of FKME was investigated on the histamine release from rat peritoneal mast cells
(RPMCs) stimulated by compound 48/80, which promotes histamine release. The human mast cell line HMC-1 was stimulated by phorbol
12-myristate 13-acetate plus calcium ionophore A23187. Activated HMC-1 can produce several proinflammatory and chemotactic
cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-8. Cytokine levels in the culture supernatant
were measured by an enzyme-linked immunosorbent assay. Cytotoxicity by FKME was determined by a 3-(4,5-dimethylthiazol-2-yl)-diphenyl-tetrazolium
bromide (MTT) assay. FKME inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling in mice. When 1 g/kg
FKME was pretreated or posttreated with mice, compound 48/80-induced mice morality was 50 and 66.7%, respectively. One gram
per kilogram of FKME pretreatment inhibited ear-swelling responses derived from compound 48/80 by 29.75%. A PCA reaction was
inhibited by 17.9%. In an in vitro model, FKME (1 mg/ml) inhibited histamine release from the RPMCs by 13.8% and TNF-α, IL-6,
and IL-8 production from HMC-1 cells by 71.16% (P < 0.001), 86.72% (P < 0.001), and 44.6%, respectively. However, FKME had no cytotoxic effects on cell viability. In conclusion, FKME inhibited
not only systemic anaphylaxis and ear swelling induced by compound 48/80 but also inhibited a PCA reaction induced by anti-DNP
IgE in vivo. Treatment with FKME showed significant inhibitory effects on histamine, TNF-α, IL-6, and IL-8 release from mast
cells. |
| |
Keywords: | Anti-allergic inflammation Compound48/80 Histamine Cytokines HMC-1 cells |
本文献已被 PubMed SpringerLink 等数据库收录! |
|