Effect of starvation and sampling time on plasma alkaline phosphatase activity and calcium homeostasis in the rat |
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| Authors: | C S Thompson D P Mikhailidis D S Gill J Y Jeremy J L Bell P Dandona |
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| Institution: | Department of Chemical Pathology and Human Metabolism, Royal Free Hospital and School of Medicine, London, UK. |
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| Abstract: | The effect of starvation and sampling time on plasma alkaline phosphatase activity, total plasma calcium concentration and whole blood ionized calcium concentration was determined in the rat. Starvation caused a significant fall in total and ionized calcium concentrations as well as in alkaline phosphatase activity. These changes were accompanied by a fall in whole blood pH and an increase in the anion gap and a decrease in urinary excretion of calcium. These indices were restored to normal following refeeding. There was no change in serum 25-OH vitamin D concentrations following starvation for 3 days. Alkaline phosphatase activity showed a pattern compatible with the presence of a circadian rhythm when sampling took place between 0800 and 1800 h. Total and ionized calcium concentrations did not show such a rhythm when animals were fed the present diet. |
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