Successful embryo transfer in Tianzhu white yak using standard protocol |
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Authors: | YU SiJiu JU XiangHong WANG LiBin & FAN JiangFeng |
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Institution: | (1) Faculty of Veterinary Medicine, Gansu Agriculture University, Lanzhou, 730070, China |
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Abstract: | The present study was carried out to investigate the efficiency of superovulation, oestrus synchronization, and embryo recovery
in Tianzhu white yaks and also to confirm the pregnancy rate of black yaks, to which embryos collected from white yaks were
transplanted. Forty-seven yaks were selected from different experiment groups, including 10 Tianzhu white female yaks (donor,
group A) and 37 black female yaks (recipient, groups B and C). Superovulation of the donor was induced by the application
procedure of CIDR-B + FSH + PG. Oestrus synchronization of recipients was induced using two methods: group B was given the
same treatment as group A, except that the follicle-stimulating hormone (FSH) injection was not administered, whereas group
C was injected with cloprostenol only once when corpus luteum (corpora lutea) was (were) palpated. The results showed that
the oestrous rates in group A were higher (80%) than those in group B (60%) and group C (44.5%). As for the efficiency of
superovulation, it was indicated that the mean numbers (±SD) of total corpora lutea, follicles, viable (transferable), and
degenerated embryos were 4.75 ± 2.19, 1.13 ± 0.83, 2.50 ± 1.31, and 1.38 ± 0.92, respectively. The mean embryo recovery rates
were 55.6%. All together, 18 viable embryos of Tianzhu white yak were obtained and 12 of them were transplanted to 10 recipients.
The pregnancy rate was 50% and the delivery rate was 40%.
Supported by Gansu Science Foundation of Application of Biological Techniques in Agriculture (Grant No. GNSW-2004-07) and
Science Foundation of Gansu Province (Grant No. 2GS042-A41-001-05) |
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Keywords: | Tianzhu white yak superovulation oestrus synchronization embryo transfer |
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