| 大鼠脑神经干细胞系(RNSC-FMU 1)的建立和鉴定 |
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| 引用本文： | 郑志竑,胡建石,林玲,游晓青,张琦,林建银. 大鼠脑神经干细胞系(RNSC-FMU 1)的建立和鉴定[J]. 分子细胞生物学报, 2004, 37(3): 199-204 |
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| 作者姓名： | 郑志竑 胡建石 林玲 游晓青 张琦 林建银 |
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| 作者单位： | 福建医科大学分子医学研究中心 福州350004(郑志竑，胡建石，林玲，游晓青，张琦)，福建医科大学分子医学研究中心 福州350004(林建银) |
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| 基金项目： | 福建省科技计划项目(2001Z020) |
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| 摘 要： | 采用无血清培养液分离和培养新生SD 大鼠脑的神经干细胞,以机械分散的方法传代,成功地建立了大鼠脑神经干细胞系(RSNC-FMU 1)。该细胞系可在体外长期传代,至今已在体外连续生长超过21个月(>100代),保持了神经干细胞的特性和正常的核型,经诱导可分化成为神经元、星形胶质细胞和少突胶质细胞,具有较旺盛的自新能力,倍增时间约为20h,并可冷冻保存,裸鼠体内移植证实其不具有成瘤性。该细胞系为神经干细胞研究提供了一个良好的工具。
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| 关 键 词： | 大鼠 神经干细胞系 建立 鉴定 |
| 修稿时间： | 2003-09-12 |
ESTABLISHMENT AND CHARACTERIZATION OF A NEURAL STEM CELL LINE (RNSC-FMU 1) FROM RAT BRAIN |
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| ZHENG Zhi Hong HU Jian Shi LIN Ling YOU Xiao Qing ZHANG Qi IJN Jian Yin. ESTABLISHMENT AND CHARACTERIZATION OF A NEURAL STEM CELL LINE (RNSC-FMU 1) FROM RAT BRAIN[J]. Journal of Molecular Cell Biology, 2004, 37(3): 199-204 |
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| Authors: | ZHENG Zhi Hong HU Jian Shi LIN Ling YOU Xiao Qing ZHANG Qi IJN Jian Yin |
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| Abstract: | A neural stem cell line (RNSC-FMU 1) From rat brain have been established suc-cessfully by isolating and culturing neural stem cells from newborn SD rat brain in vitro withfree-serum medium and passaging with mechanical division.The cell line cultured can continuous-ly generale in vitro for long-term and it is 21 months (>100 passages) so far.These cells keepthe feature of neural stem cell and normal karyotype.These neural stem cells can be induce todifferentiate into neurons,astrocytes and oligodendrocytes.The cells have an extensive self-renewalcapacity;its doubling time of proliferation is about 20h.The cells are also cryoprescrvable.Tumorformation is not observed in nude mice that explanted with the cells.This cell line in a good toolfor research of neural stem cell. |
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| Keywords: | Rat. Neural stem cell line. Establishment. Characterization |
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