The Use of Tannic Acid-Glutaraldehyde in the Study of Elastic and Elastic-Related Fibers |
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| Authors: | G Cotta-Pereira F Guerra Rodrigo J F David-Ferreira |
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| Institution: |
a Gulbenkian Institute of Science, Oeiras, Portugal
b Department of Histology and Embryology, State University of Guanabara and Federal University of Rio de Janeiro, Guanabara, Brazil
c Department of Dermatology, Faculty of Medicine, Lisbon, Portugal |
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| Abstract: | Fixation with tannic acid—glutaraldehyde permits distinction of oxytalan, elaunin and elastic fibers in the electron microscope. The results obtained using tannic acid at concentrations of 1.0%, 0.5% and 0.25% in 3% glutaraldehyde were compared. The 0.25% concentration is recommended for studying fine details of connective fibrils and for regular staining of elastin. |
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