Separation of the bovine colostrum M-1 glycoprotein into two components |
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| Authors: | Anatoly Bezkorovainy and Dietmar Grohlich |
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| Institution: | Department of Biochemistry, Presbyterian–St Luke''s Hospital, Chicago, Ill. 60612, and Department of Biological Chemistry, University of Illinois College of Medicine, Chicago, Ill. 60612, U.S.A. |
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| Abstract: | 1. Two glycoproteins were isolated from the M-1 acid glycoprotein fraction of bovine colostrum. 2. The lighter glycoprotein had a molecular weight of 7200, contained about 28.4% of carbohydrate, and had an absorption maximum at 275nm. The heavier glycoprotein had a molecular weight of 12000, contained 39.0% of carbohydrate, and had no absorption maxima in the 240-300nm. range of the spectrum. 3. The carbohydrate moiety of both glycoproteins was removable from the polypeptide moiety under the conditions of the beta-elimination reaction. 4. Periodate oxidation experiments showed that sialic acid was linked to galactose in both proteins. |
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