Cross-species microarray hybridization to identify developmentally regulated genes in the filamentous fungus <Emphasis Type="Italic">Sordaria macrospora</Emphasis> |
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Authors: | Minou?Nowrousian Carol?Ringelberg Jay?C?Dunlap Jennifer?J?Loros Email author" target="_blank">Ulrich?KückEmail author |
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Institution: | (1) Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum ND 7/131, Universitätsstr. 150, 44801 Bochum, Germany;(2) Departments of Genetics and Biochemistry, Dartmouth Medical School, Dartmouth, NH 03755, USA |
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Abstract: | The filamentous fungus Sordaria macrospora forms complex three-dimensional fruiting bodies that protect the developing ascospores and ensure their proper discharge. Several regulatory genes essential for fruiting body development were previously isolated by complementation of the sterile mutants pro1, pro11 and pro22. To establish the genetic relationships between these genes and to identify downstream targets, we have conducted cross-species microarray hybridizations using cDNA arrays derived from the closely related fungus Neurospora crassa and RNA probes prepared from wild-type S. macrospora and the three developmental mutants. Of the 1,420 genes which gave a signal with the probes from all the strains used, 172 (12%) were regulated differently in at least one of the three mutants compared to the wild type, and 17 (1.2%) were regulated differently in all three mutant strains. Microarray data were verified by Northern analysis or quantitative real time PCR. Among the genes that are up- or down-regulated in the mutant strains are genes encoding the pheromone precursors, enzymes involved in melanin biosynthesis and a lectin-like protein. Analysis of gene expression in double mutants revealed a complex network of interaction between the pro gene products. |
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Keywords: | Fungal development Differential gene expression Cross-species microarray hybridization Sordaria macrospora Neurospora crassa |
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