Heterologous expression and efficient ethanol production of a <Emphasis Type="Italic">Rhizopus</Emphasis> glucoamylase gene in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Shaohui Yang Naibing Jia Minggang Li Jiehua Wang |
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Institution: | (1) School of Agriculture and Bioengineering, Tianjin University, Tianjin, 300072, People’s Republic of China;(2) College of Life Sciences, Nankai University, Tianjin, 300071, People’s Republic of China; |
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Abstract: | Glucoamylases are inverting exo-acting starch hydrolases releasing β-glucose from the non-reducing ends of starch and related
substrates. Due to the absence of glucoamylase in Saccharomyces cerevisiae, it is not capable of utilizing starch directly as energy sources without enzymatic or chemical hydrolysis for its ethanol
production. In this study, we heterologously expressed a previously isolated Rhizopus arrhizus glucoamylase gene in S. cerevisiae host. The expressed glucoamylase enzyme was secreted into the culture supernatant and exhibited a molecular weight of 68 kDa
on SDS-PAGE gel and western blot. In the flask ferment experiment of S. cerevisiae growing on raw starch, the RaGA transformed strains could utilize starch as energy source to produce ethanol up to a final concentration as 5%. |
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