The photocycle of bacteriorhodopsin immobilized in poly(vinyl alcohol) film |
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| Authors: | K Bryl G Varo R Drabent |
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| Affiliation: | Department of Physics and Biophysics, University of Agriculture and Technology, Olsztyn, Poland. |
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| Abstract: | The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA-H2O mixture. The bacteriorhodopsin photocycle in the PVA-H2O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation to the M412-O640 and O640-bRall-trans steps. In the PVA film the O640 intermediate disappeared and a negative photoelectric signal appeared. |
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