Changes in cytoplasmic and lysosomal enzyme activities in cultured rat heart cells: The relationship to cell differentiation and cell population in culture |
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Authors: | Shu’a Yagev Michael Heller Arié Pinson |
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Institution: | (1) Laboratory for Myocardial Research, Institute of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel |
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Abstract: | Summary Postnatal rat heart cells in culture enriched with respect to muscle cells were obtained by either high density seeding or
by the replating technique. 3H]Thymidine incorporation to DNA and the enzymatic pattern of cytoplasmic and lysosomal enzymes have been studied as a function
of the culture’s age, of seeding density, and replating. It was shown that (a) replating maintains predominance of myocyte
population for at least 2 wk in culture; (b) heavy seeding density allows homogeneous myocyte population for the 1st wk in
culture; and (c) the enzyme profile of the culture may serve as an indicator for the type of cell population in culture and
its state of differentiation.
This study was done as partial fulfilment of the M.Sc. thesis in Biochemistry (SY). Supported by grants from The Chief Scientist,
Ministry of Health, State of Israel; The Ministry of Education and Sciences, State of Niedersachssen (FRG); and The Foundation
for Heart Research from Mr. and Mrs. D. Vidal-Madjar, Paris, France. |
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Keywords: | myocytes nonmuscle cells seeding density replating enzymes pattern |
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