Identification of an insertion sequence, IS1081, in Mycobacterium bovis |
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| Authors: | Desmond M Collins Diana M Stephens |
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| Institution: | Institute of Applied Microbiology, University of Tokyo, Tokyo, Japan |
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| Abstract: | Abstract: An insertion sequence, IS1081, in the genome of Mycobacterium bovis has been identified and sequenced. It is 1324 bp long with 15 bp inverted repeat ends and contains a large ORF. There are six copies of IS1081 in the genome of M. bovis and the element is also present in Mycobacterium tuberculosis . IS1081 is not closely related to other DNA elements described in actinomycetes but its putative transposase bears some resemblance to that of IS256 from Staphylococcus aureus . IS1081 may be useful for genetic manipulations and for developing a diagnostic test for bovine tuberculosis based on the polymerase chain reaction. |
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| Keywords: | Chromate reduction Electron acceptor Respiratory system Anaerobic membrane vesicle Reduced minus oxidized spectrum |
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