Evaluation of the new advanced 15-loci MIRU-VNTR genotyping tool in Mycobacterium tuberculosis molecular epidemiology studies |
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| Authors: | Noelia Alonso-Rodríguez Miguel Martínez-Lirola Marta Herránz Marisa Sanchez-Benitez Pilar Barroso INDAL-TB group Emilio Bouza Darío García de Viedma |
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| Affiliation: | 1.Servicio de Microbiología y Enfermedades Infecciosas. Hospital Gregorio Mara?ón,Universidad Complutense, Madrid, CIBER de Enfermedades Respiratorias (CIBERES),Spain;2.Complejo Hospitalario Torrecárdenas,Almería,Spain;3.Unidad de Tuberculosis de Poniente,Almería,Spain;4.Delegación de Salud Distrito Levante,Almería,Spain |
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| Abstract: | Background During the last few years, PCR-based methods have been developed to simplify and reduce the time required for genotyping Mycobacterium tuberculosis (MTB) by standard approaches based on IS6110-Restriction Fragment Length Polymorphism (RFLP). Of these, MIRU-12-VNTR (Mycobacterial interspersed repetitive units- variable number of tandem repeats) (MIRU-12) has been considered a good alternative. Nevertheless, some limitations and discrepancies with RFLP, which are minimized if the technique is complemented with spoligotyping, have been found. Recently, a new version of MIRU-VNTR targeting 15 loci (MIRU-15) has been proposed to improve the MIRU-12 format. |
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