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New Views of Old Proteins: Clarifying the Enigmatic Proteome
Authors:Kristin E Burnum-Johnson  Thomas P Conrads  Richard R Drake  Amy E Herr  Ravi Iyengar  Ryan T Kelly  Emma Lundberg  Michael J MacCoss  Alexandra Naba  Garry P Nolan  Pavel A Pevzner  Karin D Rodland  Salvatore Sechi  Nikolai Slavov  Jeffrey M Spraggins  Jennifer E Van Eyk  Marc Vidal  Christine Vogel  Neil L Kelleher
Institution:1. The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA;2. Inova Women’s Service Line, Inova Health System, Falls Church, Virginia, USA;3. Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina, USA;4. Department of Bioengineering, University of California, Berkeley, California, USA;5. Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, New York, USA;6. Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, USA;7. Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden;8. Department of Genome Sciences, University of Washington, Seattle, Washington, USA;9. Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois, USA;10. Department of Pathology, Stanford University, Stanford, California, USA;11. Department of Computer Science and Engineering, University of California at San Diego, San Diego, California, USA;12. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA;13. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA;14. Department of Bioengineering, Northeastern University, Boston, Massachusetts, USA;15. Department of Cell and Developmental Biology, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA;16. Advanced Clinical Biosystems Institute in the Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, California, USA;17. Department of Genetics, Harvard University, Cambridge, Massachusetts, USA;18. New York University Center for Genomics and Systems Biology, New York University, New York, New York, USA;19. Department of Pathology, Harvard Medical School, Brigham and Women’s Hospital, Wyss Institute at Harvard University, Boston, Massachusetts, USA;20. Department of Chemistry, Northwestern University, Evanston, Illinois, USA
Abstract:All human diseases involve proteins, yet our current tools to characterize and quantify them are limited. To better elucidate proteins across space, time, and molecular composition, we provide a >10 years of projection for technologies to meet the challenges that protein biology presents. With a broad perspective, we discuss grand opportunities to transition the science of proteomics into a more propulsive enterprise. Extrapolating recent trends, we describe a next generation of approaches to define, quantify, and visualize the multiple dimensions of the proteome, thereby transforming our understanding and interactions with human disease in the coming decade.
Keywords:proteins  proteomics  single-cell biology  biotechnology  single-molecule sequencing  ECM"}  {"#name":"keyword"  "$":{"id":"kwrd0040"}  "$$":[{"#name":"text"  "_":"extracellular matrix  HuBMAP"}  {"#name":"keyword"  "$":{"id":"kwrd0050"}  "$$":[{"#name":"text"  "_":"Human BioMolecular Atlas Program  MS"}  {"#name":"keyword"  "$":{"id":"kwrd0060"}  "$$":[{"#name":"text"  "_":"mass spectrometry  PTM"}  {"#name":"keyword"  "$":{"id":"kwrd0070"}  "$$":[{"#name":"text"  "_":"post-translational modification
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