Analysis of Con A-binding glycoproteins in synaptosomal membranes |
| |
Authors: | Noriaki Ishioka Susumu Kurioka |
| |
Institution: | (1) Division of Biochemistry, Institute of Medical Science, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105 Minato-ku, Tokyo, Japan |
| |
Abstract: | Concanavalin A (Con A)-binding proteins obtained from solubilized synaptosomal membranes of bovine brain were analyzed by two-dimensional electrophoresis (2DE), and were identified by peroxidase conjugated Con A (Con A-peroxidase staining), after transfer from 2DE gel to nitrocellulose paper. The Con A-binding proteins were resolved up to 40 spots, ranging in isoelectric points (pI) from 4.5 to 8.0 and molecular weight (MW) from 10 kDa to 120 kDa. Most of the Con A-binding proteins were streaked across a pH gradient and/or exhibited as multiple spots, indicating broad charge and molecular weight heterogeneity. The presence of protein groups that showed high affinities for Con A were revealed. Most interesting group (named GP51), which consisted of seven spots separated horizontally in charge heterogeneity (pI5.85-7.5) with MW 51kDa, was characterized by its binding to an immobilized protein A gel. This implies that GP51 is related to immunoglobulins and/or GP51 may be a new member of the immunoglobulin supergene family. |
| |
Keywords: | Synaptosomes membrane glycoproteins Con A-binding protein A-binding two-dimensional electrophoresis immunoglobulin supergene family |
本文献已被 SpringerLink 等数据库收录! |
|