Antigen-antibody complexes stimulate the synthesis and release of prostaglandins by mouse peritoneal macrophages |
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Authors: | Robert J Bonney Peter Naruns Philip Davies John L Humes |
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Institution: | Departments of Immunology and Biochemistry of Inflammation Merck Institute for Therepeutic Research P.O. Box 2000 Rahway, New Jersey 07065 USA |
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Abstract: | Antigen-antibody complexes (Ag/Ab) formed at equivalence stimulate the release of arachidonic acid and synthesis of prostaglandin E2 and 6-keto-prostaglandin F1α by resident mouse peritoneal macrophages. Prostaglandin synthesis and secretion is stimulated by submicrogram quantities of Ag/Ab which increases in a dose-dependent manner. This release is time-dependent and occurs in the absence of any loss of cell viability as indicated by increased cellular levels of lactate dehydrogenase without concomitant loss of this activity to the media and the continued secretion of a constitutive cellular product, lysozyme. The stimulated synthesis of prostaglandins by Ag/Ab is inhibited by indomethacin and physiological levels of antiinflammatory glucocorticoids. |
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