Carbohydrate moieties of peanut agglutinin receptors isolated from human gastric cancer cells |
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| Authors: | N Shimoda H Muramatsu T Osawa T Yamada M Adachi T Muramatsu |
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| Affiliation: | Department of Biochemistry, Faculty of Medicine, Kagoshima University. |
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| Abstract: | Receptors for peanut agglutinin (PNA) were isolated from Kato III human gastric cancer cells by affinity chromatography on PNA agarose, and were labeled by the galactose oxidase-NaB3H4 method. Alkaline NaBH4 treatment of the labeled receptors released two small oligosaccharide alcohols, which were identified as Gal beta 1----3GalNAc-ol and Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAc-ol. Higher oligosaccharides and glycopeptides of both N- and O-linked type were also detected, but they did not appear to bear PNA binding sites. The presence of oligo-N-acetyllactosamine units in the N-linked type sugars was indicated by endo-beta-galactosidase digestion. |
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