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Ionol (BHT) produces superoxide anion
Authors:Smirnova E G  Lyubimov Yu I  Malinina T G  Lyubimova E Yu  Alexandrushkina N I  Vanyushin B F  Kolesova G M  Yaguzhinsky L S
Institution:(1) Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Timiryazevskaya ul. 42, Moscow, 127550, Russia;(2) Scientific-Production Complex ldquoBiotechindustryrdquo, 2-i Spaso-Nalivkovsky Pereulok 6, Moscow, 119991, Russia;(3) Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899, Russia
Abstract:In aqueous medium etiolated wheat seedlings release superoxide anion ( 
$$O_2^{_ \cdot ^ -}$$
). Interaction of a synthetic antioxidant, butylated hydroxytoluene (BHT, ionol), with oxygen in the aqueous medium is accompanied by 
$$O_2^{_ \cdot ^ -}$$
formation. This suggests that under certain conditions BHT behaves as a prooxidant. A natural antioxidant, superoxide dismutase (SOD), and also a wound healing preparation, emulsified denatured placenta (EDP), do not exhibit the prooxidant properties. In contrast to BHT, they reduce 
$$O_2^{_ \cdot ^ -}$$
production by the etiolated wheat seedling system.
Keywords:BHT  antioxidant  prooxidant  wheat seedlings  reactive oxygen species  superoxide
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