| 蟹爪兰茎段消毒及其丛生芽增殖和生根初步研究 |
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| 引用本文： | 尹明华,戴琴琴,张洁,刘林妹,吴树英.蟹爪兰茎段消毒及其丛生芽增殖和生根初步研究[J].亚热带植物科学,2010,39(3):24-26. |
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| 作者姓名： | 尹明华 戴琴琴 张洁 刘林妹 吴树英 |
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| 作者单位： | 上饶师范学院,生命科学学院,江西,上饶,334001 |
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| 基金项目： | 江西省教育厅科技一般项目，上饶师范学院2010-2011年度院级科技项目 |
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| 摘 要： | 以蟹爪兰茎段为材料,采用组织培养和单因子实验方法,研究蟹爪兰无菌体系的建立及植物生长调节剂对茎段丛生芽增殖和生根的影响。结果表明:蟹爪兰茎段最佳消毒方式是75%酒精30 s+0.1%HgCl2 10 min;茎段丛生芽增殖最佳培养基是MS+KT 4 mg/L+NAA 0.1 mg/L;生根最佳培养基是1/2MS+NAA 0.5 mg/L。
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| 关 键 词： | 蟹爪兰 茎段 消毒方式 丛生芽 增殖 生根 |
Preliminary Study on Stem Disinfection of Zygocactus truncactus and Its Multiple Bud Proliferation and Rooting |
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| YIN Ming-hua,DAI Qin-qin,ZHANG Jie,LIU Lin-mei,WU Shu-ying.Preliminary Study on Stem Disinfection of Zygocactus truncactus and Its Multiple Bud Proliferation and Rooting[J].Subtropical Plant Science,2010,39(3):24-26. |
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| Authors: | YIN Ming-hua DAI Qin-qin ZHANG Jie LIU Lin-mei WU Shu-ying |
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| Institution: | YIN Ming-hua,DAI Qin-qin,ZHANG Jie,LIU Lin-mei,WU Shu-ying(College of Life Science,Shangrao Normal University,Shangrao 334001,Jiangxi China) |
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| Abstract: | The establishment of sterile system and the effect of plant growth regulators on multiple bud proliferation and rooting were studied by tissue culture and single-factor experimental method,using Zygocactus truncactus stems as material.The results showed that the best sterilization method of Z.truncactus stems was firstly treated with 75% alcohol for 30s and then treated with 0.1% HgCl2 for 10min,the best multiple bud proliferation medium of Z.truncactus was MS + KT 4 mg/L + NAA 0.1 mg/L,and the best rooting... |
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| Keywords: | Zygocactus truncactus stem sterilization method multiple bud proliferation rooting |
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