Growth Kinetics of <Emphasis Type="Italic">Lactococcus lactis</Emphasis> ssp. <Emphasis Type="Italic">diacetylactis</Emphasis> Harboring Different Plasmid Content |
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Authors: | Kibeom Lee Seung-Hyeon Moon |
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Institution: | (1) Department of Environmental Science and Engineering, Kwangju Institute of Science and Engineering, Kwangju, South Korea, KR |
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Abstract: | The effect of plasmid content on growth of Lactococcus lactis ssp. diacetylactis harboring different plasmids and on plasmid stability was studied. Strain DRC-2C is a plasmid Lac+- and Prt+-free strain. Strain DRC-2 utilizes lactose as carbohydrate and has proteinase activity. The plasmid-free strain DRC-2C exhibited
none of these features. Plasmid-encoded properties were clearly identified. Results showed that plasmid content decreased
bacterial growth in terms of the specific growth rate determined. Slightly lower specific growth rate and lactic acid production
were observed in the strain of higher plasmid content owing to the plasmid presence, causing metabolic burden to the host
cell. The plasmid profile results showed that the number of bands in the two strains before and after fermentation were the
same. This indicated that the plasmids were stably maintained and unchanged during the fermentation.
Received: 27 July 2002 / Accepted: 27 August 2002 |
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