Normal or Retrovirus-Infected Cultured Chicken Embryo Cells Express the 48,000 D Age-Related Antigen Characteristic of Embryonic Chicken Erythrocytes |
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| Authors: | O PERISIC J M BIQUARD J P BLANCHET T B GREENLAND V KRSMANOVIC |
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| Institution: | Unitéde Virologie Fondamentale et Applique INSERM - U51, 1, place Pr Joseph Renaut, 69371 Lyon Cedex 2, France;Institut du Radium, Facultédes Sciences, Bâtiment 110, 91400 Orsay, France;Département de Biologic Générale et Appliquée, UniversitéClaude Bernard, 69621 Villeurbanne, France. |
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| Abstract: | The surface of normal or retrovirus-infected chick embryo cells was labelled with 125I using lactoperoxidase. The solubilized membrane material was allowed to react with antisera raised in rabbits to cultured chick embryo cells or to the membranes of embryonic or adult chicken erythrocytes. Analysis of the immunoprecipitates shows that chicken embryo cells not of erythropoietic origin express on their surface membrane an antigenic polypeptide of mol. wt. 48,000 daltons (D), which appears to be identical with that expressed on embryonic chicken erythrocytes. |
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