Differentiating Type i and Type ii Alveolar Cells in Rat Lung by OsO4NaI Staining |
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Authors: | William F McNary JR Al-Walid El-Bermani |
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Institution: |
a Boston University School of Medicine, Boston, Massachusetts |
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Abstract: | The fixing-staining mixture consisted of 1 part of 2% aqueous OsO4 and 3 parts of 3% Nal in distilled water. Fresh lungs were cut into 2 mm slices and immersed in this solution for 24 hr at room temperature. Controls were fixed in buffered OsO4 alone. Selective staining of type II alveolar cells was shown by the OsOt-NaI mixture but was absent in the controls. No additional staining of the sections was required, and the selectivity was readily observable in either paraffin or Araldite sections by light microscopy and in Araldite sections by electron microscopy |
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