Preparation of solubilized insulin receptors from human lymphocytes |
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| Authors: | J R Gavin D N Buell J Roth |
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| Affiliation: | 1. Section on Diabetes and Intermediary Metabolism Clinical Endocrinology Branch National Institute of Arthritis, Metabolism, and Digestive Diseases National Institutes of Health Bethesda, Maryland 20014 USA;2. Immunology Branch National Cancer Institute National Institutes of Health Bethesda, Maryland 20014 USA |
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| Abstract: | Specific insulin receptors from human lymphocytes in culture have been prepared using the nonionic detergent NP-40. Receptors were solubilized from intact cells and from crude plasma membrane fractions. Insulin receptors prepared in this manner retain many of the characteristics of the receptors studied in intact cells. |
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