Plant regeneration from callus culture of a Paphiopedilum hybrid |
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Authors: | Lin Yung-Haw Chang Chen Chang Wei-Chin |
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Institution: | (1) Institute of Botany, Academia Sinica, Taipei, Taiwan, 115, epublic of China |
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Abstract: | Totipotent calli of a Paphiopedilum hybrid (Paphiopedilum callosum ‘Oakhi’ × Paph. lawrenceanum ‘Tradition’) were induced from seed-derived protocorms on a 1/2 strength Murashige–Skoog medium plus 1–10 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1–1 mg l−1 1-phenyl-3-(1.2.3-thiadiazol-5-yl)urea (TDZ). These calli grew well when subcultured on the same medium, but proliferated
more on 1/2 MS medium plus 5 mg l−1 2,4-D and 1 mg l−1 TDZ. Calli developed further along a route of production of protocorm-like bodies and eventually formed plantlets that could
be transplanted to pots and grew well.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | callus proliferation Paphiopedilum plant regeneration protocorm formation totipotent callus |
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