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Characterization of a site-specific restriction endonuclease SphI from Streptomyces phaeochromogenes |
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| Authors: | LY Fuchs L Covarrubias L Escalante S Sanchez F Bolivar |
| Institution: | Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México 20, D.F., Apdo. Postal 70228, Mexico |
| Abstract: | A new type-II restriction endonuclease SphI, has been partially purified from Streptomyces phaeochromogenes. SphI recognizes the hexanucleotide sequence 5′-GCATG↓C and cleaves it at the position marked by the arrow. This nucleotide sequence is present twice in SV40 DNA, four times in λ DNA and only once in the cloning vehicles pBR322, pBR325, pBR327 and pBR328. |
| Keywords: | Type II endoR DNase 5′-GCATG ↓ C hexanucleotide site: recombinant DNA plasmid pBR322 pBR327 RBR328 cloning vehicles EB extract buffer PMSF phenylmethyl-sulfonyl fluoride TB Tris buffer |
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