Plant regeneration from cell suspension-derived protoplasts of Saintpaulia ionantha Wendl |
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Authors: | Yoichiro Hoshino Masaru Nakano Masahiro Mii |
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Institution: | (1) Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University, 648 Matsudo, 271 Chiba, Japan;(2) Present address: Graduate School of Science and Technology, Niigata University, Japan |
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Abstract: | Summary Friable calli were induced on leaf segments of Saintpaulia ionantha Wendl. on B5 medium containing 1 mg l–1 2,4-D and 2 g l–1 casein hydrolysate. Cell suspension cultures were readily established from these friable calli and protoplasts could be isolated from the cells with yields of 1–3×107/g f. wt.. By culturing in 0.1 % gellan gum-solidified B5 medium supplemented with 1 mg l–1 2,4-D and 0.1 M each of sucrose and mannitol at a density of 1×105/ml, the protoplasts divided within 6 days and formed macro-colonies after 2 months of culture. Shoot regeneration from protoplast-derived calli was obtained by sequential treatment of the calli with plant growth regulators: initially with 1 mg l–1 each of NAA and BA for 2 months followed by 0.01 mg l–1 NAA and 5 mg l–1 BA for 4 months. Regenerated plants were established after rooting of the shoots on half-strength MS medium, and successfully transferred to the greenhouse. The regenerated plants grew into flowering stage and showed the same phenotype as the parent plant.Abbreviations BA
benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- FDA
fluorescein diacetate
- f. wt
fresh weight
- MES
2-(N-morpholino)-ethanesulfonic acid
- MS
Murashige and Skoog (1962)
- NAA
-naphthaleneacetic acid
- PE
plating efficiency |
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