cDNA cloning and expression of an apoptosis-related gene, human TFAR15 gene

By means of eDNA-RDA)A method, some cd)YA fragment- were found to have high levels oi expression during deprivation of GM-CSF ( granulocyte macrophage-rolony stimulating (actor) in a human myeloid cell line, TF-I cells. One of these fragments was identified as a novel gene. To gel the full length of eDNA, rapid amplification of cDYA ends (RACE) and expressed sequence tags ( EST) overlapting fragments assembling strategies were used. The novel gene was named TRAF15 (TF-1 cell apoptosis related gene-15) , which consist of I 218 nucleotides and eneodes 212 ammo acids. The putative protein product oi TFAR15 is parlially homologous to C. elegans protein C14A4. 11 . TFAR15 mRNA is expressed in fetal liver, kidney, spleen and lung. and also in some human myeloid cell lines. Both of the TFAR15 mRNA and protein in wen: highly expressed in TF-1 cells after GM-CSF withdrawal. In vitro analysis showed that the tecombinant TFAR15 protem coold inhibit the natural cell death of 293 cells, an embryonie kidney cell line.