Accumulation of fructose 1,6-bisphosphate in mutant cells of mucoid Pseudomonas aeruginosa as an evidence of phosphofructokinase activity |
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| Authors: | P C Banerjee |
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| Institution: | (1) Department of Microbiology and Immunology, University of Illinois Health Sciences Center, 60612 Chicago, IL, USA;(2) Present address: Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032 Calcutta, India |
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| Abstract: | Phosphoglucose isomerase negative mutant of mucoid Pseudomonas aeruginosa accumulated relatively higher concentration of fructose 1,6-bisphosphate (Fru-1,6-P2) when mannitol induced cells were incubated with this sugar alcohol. Also the toluene-treated cells of fructose 1,6-bisphosphate aldolase negative mutant of this organism produced Fru-1,6-P2 from fructose 6-phosphate in presence of ATP, but not from 6-phosphogluconate. The results together suggested the presence of an ATP-dependent fructose 6-phosphate kinase (EC 2.7.1.11) in mucoid P. aeruginosa.Abbreviations ALD
Fru-1,6-P2 aldolse
- DHAP
dihydroxyacetone phosphate
- F6P
fructose 6-phosphate
- G6P
glucose 6-phosphate
- Gly3P
glyceraldehyde 3-phosphate
- KDPG
2-keto 3-deoxy 6-phosphogluconate
- PFK
fructose 6-phosphate kinase
- PGI
phosphoglucose isomerase
- 6PG
6-phosphogluconate |
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| Keywords: | Pseudomonas aeruginosa Fructose 6-phosphate kinase Fructose bisphosphate aldolase mutant Phosphoglucose isomerase mutant Fructose bisphosphate formation Mucoid |
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