Stable isotope labelled 5-lipoxygenase metabolites of arachidoic acid: Analysis by negative ion chemical ionization mass spectrometry |
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| Affiliation: | 1. Instituto de Física Enrique Gaviola - CONICET, Av. M. Allende s/n, 5000 Córdoba, Argentina;2. Laboratorio de Investigación e Instrumentación en Física Aplicada a la Medicina e Imágenes por Rayos X, Universidad Nacional de Córdoba, Av. M. Allende s/n, 5000 Córdoba, Argentina;3. Centro de Física e Ingeniería en Medicina - CFIM & Departamento de Ciencias Físicas, Universidad de La Frontera, Av. Francisco Salazar 1145, Casilla 54-D, Temuco, Chile;4. Departamento de Física, Universidad Nacional, Heredia, Costa Rica, Avenida 1, Calle 9. Apartado Postal: 86-3000, Heredia, Costa Rica;5. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica, Córdoba, Argentina;6. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada, IPQA, CONICET, Córdoba, Argentina;7. ICOS Inmunomédica, Lago Puyehue, 1750 Temuco, Chile;8. Facultad de Ciencias, Escuela de Tecnología Médica, Universidad Mayor, Chile;1. Department of Mathematics, Air University Sector E-9 AIR Complex, P. O. 44000, Islamabad, Pakistan;2. Faculty of Mathematics and Statistics, Ton Duc Thang University, Ho Chi Minh City 72915, Viet Nam;3. Department of Mathematics, College of Arts and Sciences, Prince Sattam bin Abdulaziz University, Wadi Aldawaser 11991, Saudi Arabia;1. Jubilant Biosys Limited, B-34, Sector-58, Noida, UP 201301, India;2. Department of Chemistry, Gautam Buddha University, Greater Noida, UP 20130, India;1. Institute of Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russian Federation;2. Institute of Problems of Chemical Physics, Russian Academy of Sciences, 142432, Chernogolovka, Russian Federation;3. Scientific Center of the Russian Academy of Sciences, 142432, Chernogolovka, Russian Federation;4. Moscow State University, 119992, Moscow, Russian Federation;5. Russian National Research Medical University, 119997, Moscow, Russian Federation;1. Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba 278-8510, Japan;2. Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, 1 Sanzo, Gakuen-cho, Fukuyama-shi, Hiroshima 729-0292, Japan;3. Department of Biochemistry, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582, Japan;4. Endocrinology & Diabetes Center, Yokohama Rosai Hospital, 3211 Kozukue-Cho, Kohoku-Ku, Yokohama 222-0036, Japan;5. Department of Uro-Oncology, Saitama Medical University International Medical Center, 1397-1 Yamane, Hidaka-shi, Saitama 350-1298, Japan |
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| Abstract: | ![]()
Purification and analysis of very small quantities of arachidonic acid lipoxygenase products from biological samples remains a challenging task. Gas chromatography-mass spectrometry is an ideally suited technique unsurpassed in the analytical figures of merit of sensitivity, specificity, and accuracy for the quantitation of such hydroxy lipids as 5-hydroxyeicosatetraenoic acid and the LTB4 isomers. We have developed procedures to incorporate oxygen-18 into the carboxyl moiety of these fatty acids as well as 12-hydroxyl position of LTB4 and report on the suitability of these isotopimers as internal standards for quantitative mass spectrometry. Furthermore, the enhanced sensitivity of negative ion chemical ionization of the pentafluorobenzyl ester, trimethylsilyl ether derivatives has been examined. The most abundant ion obtained by this technique is the carboxylate anion which retains all oxygen atoms of these eicosanoids. Comparison of the positive electron impact and negative ion CI mass spectrometric behavior is presented. |
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