<Emphasis Type="Italic">Xerophyta viscosa</Emphasis> Aldose Reductase,XvAld1, Enhances Drought Tolerance in Transgenic Sweetpotato |
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Authors: | Wilton Mbinda Omwoyo Ombori Christina Dixelius Richard Oduor |
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Institution: | 1.Department of Biochemistry and Biotechnology,Pwani University,Kilifi,Kenya;2.Department of Biochemistry and Biotechnology,Kenyatta University,Nairobi,Kenya;3.Department of Plant Sciences,Kenyatta University,Nairobi,Kenya;4.Department of Plant Biology,Swedish University of Agricultural Sciences,Uppsala,Sweden |
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Abstract: | Sweetpotato is a significant crop which is widely cultivated particularly in the developing countries with high and stable yield. However, drought stress is a major limiting factor that antagonistically influences the crop’s productivity. Dehydration stress caused by drought causes aggregation of reactive oxygen species (ROS) in plants, and aldose reductases are first-line safeguards against ROS caused by oxidative stress. In the present study, we generated transgenic sweetpotato plants expressing aldose reductase, XvAld1 isolated from Xerophyta viscosa under the control of a stress-inducible promoter via Agrobacterium-mediated transformation. Our results demonstrated that the transgenic sweetpotato lines displayed significant enhanced tolerance to simulated drought stress and enhanced recuperation after rehydration contrasted with wild-type plants. In addition, the transgenic plants exhibited improved photosynthetic efficiency, higher water content and more proline accumulation under dehydration stress conditions compared with wild-type plants. These results demonstrate that exploiting the XvAld1 gene is not only a compelling and attainable way to improve sweetpotato tolerance to drought stresses without causing any phenotypic imperfections but also a promising gene candidate for more extensive crop improvement. |
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