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Isolation and chromosomal localization of ecdysterone-responsive genes in a Drosophila cell line |
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| Authors: | J. L. Couderc J. L. Becker M. L. Sobrier B. Dastugue M. Best-Belpomme J. A. Lepesant M. L. Pardue |
| Affiliation: | (1) Faculté de Médecine, Laboratoire de Biochimie Médicale, 28, place Henri-Dunant, 63001 Clermont-Ferrand Cedex, France, CNRS LA 360;(2) Laboratoire de Zoologie, Université Pierre et Marie Curie, 75230 Paris cedex 05, France;(3) Département de Différenciation Cellulaire et Moléculaire, Institut Jacques Monod, Université Paris VII, 75231 Paris cedex 05, France;(4) Department of Biology, Massachusetts Institute of Technology, 02139 Cambridge, MA, USA |
| Abstract: | Cultured Kc 0% cells of Drosophila melanogaster are responsive to ecdysterone treatment. A library of lambda phages carrying segments of Drosophila genomic DNA was differential screened using poly(A)+RNAs from control and ecdysterone-treated cells. Nine independent recombinant phages that hybridized more intensely with poly(A)+ RNA from treated cells and six that hybridized most strongly with poly(A)+RNA from untreated cells were selected. Genomic localization of these  inducible and repressible sequences was determined by hybridization in situ. These results suggest that expression of several unique genes is increased by the hormone. The six repressible sequences each contained DNA that hybridized to multiple chromosomal sites and appeared to be mobile elements, suggesting that the steroid hormone might be acting on the transposable elements. These probes will be useful for the study of positive and negative steroid regulation within the same cell. |
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