| Tet调控的EB病毒LMP1基因导入鼻咽癌细胞系表达的研究 |
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| 引用本文： | 廖伟,曹亚.Tet调控的EB病毒LMP1基因导入鼻咽癌细胞系表达的研究[J].生物化学与生物物理学报,1999,31(3):309-312. |
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| 作者姓名： | 廖伟 曹亚 |
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| 作者单位： | 湖南医科大学肿瘤研究所 |
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| 基金项目： | 国家自然科学基金,国家自然科学杰出青年基金,中华医学基金 |
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| 摘 要： | 本文利用Ｔｅｔ－ｏｎ基因表达系统建立了一株可诱导ＥＢ病毒ＬＭＰ１基因表达的鼻咽癌细胞株。首先将Ｔｅｔ－ｏｎ基因调控系统的调节质粒ｐＴｅｔ－ｏｎ导入鼻咽癌细胞系ＨＮＥ２中，用ｒｔＴＡ反应性芝光素酶报道基因ｐＴＲＥ－ｌｕｃ挑选高诱导、低背景的克隆，第二轮转染将构建的反应质粒ｐＴＲＥ－ＬＭＰ１导入得到稳定转染的阳性克隆，对各克隆用Ｗｅｓｔｅｒｎ印迹方法进行强力霉素诱导效应检测，其中Ｌ７对Ｄｏｘ具有良好的
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| 关 键 词： | EB病毒 潜伏膜蛋白 基因 诱导表达 鼻咽癌 |
Doxycycline Dependent Expression of Epstein Barr Virus Latent Membrane Protein 1 with Tet Regulating System in Nasopharygeal Carcinoma Cell Line |
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| LIAO Wei,YI Hong,LI Xiao Yan,TANG Ming,GU Huan Hua and CAO Ya.Doxycycline Dependent Expression of Epstein Barr Virus Latent Membrane Protein 1 with Tet Regulating System in Nasopharygeal Carcinoma Cell Line[J].Acta Biochimica et Biophysica Sinica,1999,31(3):309-312. |
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| Authors: | LIAO Wei YI Hong LI Xiao Yan TANG Ming GU Huan Hua and CAO Ya |
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| Institution: | LIAO Wei,YI Hong,LI Xiao Yan,TANG Ming,GU Huan Hua and CAO Ya * |
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| Abstract: | Latent membrane protein 1(LMP1) of Epstein Barr virus (EBV) plays a vital role in cell transformation and the nasopharygeal carcinoma (NPC) is highly related to EBV infection. To elucidate whether LMP1 exerts oncogenic effect in NPC and its possible mechanism, a dual stable LMP 1 integrated NPC cell line with Tet on regulating system were established. Results revealed that expression of LMP 1 could be regulated by Tet system when NPC cell line HNE 2 served as receipt cell, and doxycycline could increase this expression by about 10 fold. Therefore, it is possible that different level expression of LMP 1 results in its different biological functions and various efforts on nasopharyngeal carcinogenesis. |
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| Keywords: | Epstein Barr virus latent membrane protein 1 regulated expression nasopharyngeal carcinoma |
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