毛细管区带电泳／串联质谱联用法鉴定多肽和蛋白质

建立了毛细管区带电泳－串联质谱联用（ＣＺＥ／ＭＳ／ＭＳ）对多肽和蛋白质高灵敏度鉴定方法，对Ｍｅｔ－脑啡肽和Ｌｅｕ－脑啡肽的混合物进行了分析，用ＣＺＥ／ＭＳ／ＭＳ方法验证了各自的序列，同样对细胞色素ｃ的胰蛋白酶酶解产物用ＣＺＥ／ＭＳ／ＭＳ方法进行了肽质谱分析，几科所有肽段的序列及其与在分子中的位置都得到了确定，通过ＳＥＱＵＥＳＴ软件进行蛋白质序列数据库搜索得到准确的鉴定结果，所消耗的样品量均在低皮可

Protein and Peptide Identification by Capillary Zone Electrophoresis Tandem Mass Spectrometry

A method for highly sensitive protein and peptide identification by capillary zone electrophoresis/electrospray ionization tandem mass spectrometry (CZE/ESI/MS/MS) was described. A mixture of peptides Met enkephalin and Leu enkephalin was analyzed, and their amino acid sequences were identified, respectively, using CZE/MS/MS. Peptide mapping for a tryptic digest of horse cytochrome c was performed by the same method. The results indicated that almost all peptides generated by tryptic digestion were identified, and their sequences were interpreted by their collision induced (CID) mass spectra. Applying the uninterpreted CID spectra of the protein digest to searching in sequence database by the SEQUEST program could identify proteins conveniently and rapidly. The samples consumed in the CZE/MS/MS experiments were at picomole level, so this method is quite suitable for quality control of recombinant proteins and trace amount protein identification in the proteome research.