Systematic cross-validation of 454 sequencing and pyrosequencing for the exact quantification of DNA methylation patterns with single CpG resolution |
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| Authors: | Anna Potapova Cord Albat Britta Hasemeier Katrin Haeussler Stella Lamprecht Sebastian Suerbaum Hans Kreipe Ulrich Lehmann |
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| Institution: | (1) Institute of Pathology, Medizinische Hochschule Hannover, D-30625 Hannover, Germany;(2) Institute of Biometrics, Medizinische Hochschule Hannover, D-30625 Hannover, Germany;(3) Institute of Medical Microbiology and Hospital Epidemiology, Medizinische Hochschule Hannover, D-30625 Hannover, Germany |
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| Abstract: | Background New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns
in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods
is not yet available. Therefore, a systematic cross-validation of 454 sequencing and conventional pyrosequencing, both of
which offer exact quantification of methylation levels with a single CpG dinucleotide resolution, was performed. |
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