信息素信号通路基因在斑玉蕈生长发育过程中的差异表达

为了更清楚地了解斑玉蕈菌丝成熟、原基形成和子实体发育的过程,本研究对不同菌丝培养时期的栽培瓶进行出菇实验,并对其不同培养时期和生长发育关键时期的信息素通路基因进行差异表达分析,以期揭示信息素信号通路基因参与调节斑玉蕈菌丝的生长、子实体形成和发育的作用。研究结果表明：斑玉蕈菌丝培养40-80d过程中,子实体产量呈上升的趋势,说明菌丝的成熟程度对产量会产生重要影响。对斑玉蕈基因组中的信息素信号通路基因进行分析鉴定共获得了8个关键基因。信息素通路基因差异表达分析表明：在菌丝培养40-80d过程中,大部分信息素信号通路基因在第60天时表达量最高,其中ste20、cdc24和ste12上调了4-20倍,而在第80天出现下降。从菌丝恢复到扭结形成原基和子实体发育的过程中,大多数基因在原基时期表达量最高,其中ste20、cdc24、ste11和ste12表达量上调最为显著,在子实体成熟期这些基因表达量下降。因此,这说明在菌丝营养生长过程中,在第60天菌丝细胞增殖生长最为旺盛,而在第80天菌丝细胞基本停止生长,菌丝也逐渐达到成熟。同时,在菌丝生殖生长过程中,斑玉蕈持续地上调信息素通路基因表达使菌丝细胞不断地分裂增殖,从而使新生的菌丝扭结形成原基,其中ste3、ste20、cdc24、ste11和ste12基因可能对斑玉蕈菌丝细胞的分裂增殖和诱导子实体形成起到关键的作用。

Differential expression of pheromone signal pathway genes during Hypsizygus marmoreus growth and development

Fruiting experiment of different mycelial growth stages of Hypsizygus marmoreus in bottled cultivation was carried out, and differential expression of pheromone pathway genes in different cultivation stages and key growth stages was investigated for revealing the role of pheromone signaling pathway genes in regulating the mycelial maturation, primordia formation and fruiting body development of the fungus. The results show that during 40-80 day mycelial cultivation, the fruit body production trends to upward tendency, indicating that the mycelium maturity has an important impact on the production. A total of eight key genes was obtained by analyzing and identifying the pheromone signaling pathway genes in the H. marmoreus genome. The differential expression analysis of pheromone pathway genes showed that during 40-80 day mycelial culture, most of the pheromone signaling pathway genes had the highest expression levels on the 60th day, of which ste20, cdc24, and ste12 were up-regulated 4-20 times, but decreased on the 80th day. In the process of mycelial regeneration, primordium formation and fruiting body development, most of the genes had the highest expression in the primordium stage and the expression decreased in the fruiting stage, of which the expression levels of ste20, cdc24, ste11 and ste12 were most significantly up-regulated. This indicated that the mycelial cells proliferated most vigorously on the 60th day, and began stagnancy on the 80th day, and then the mycelia gradually matured. During reproductive growth, the fungus continuously up-regulates the pheromone pathway gene expression to multiply the mycelial cells for primordium formation, in which the genes ste3, ste20, cdc24, ste11 and ste12 might play key role in proliferation of mycelia and the formation of fruiting bodies.