Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae |
| |
| Authors: | Akinori Matsushika Hiroyuki Inoue Katsuji Murakami Osamu Takimura Shigeki Sawayama |
| |
| Institution: | 1. CEB – Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal;2. Biorefinery and Food Engineering Laboratory, Food Research Department/School of Chemistry, Autonomous University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés, 25280 Saltillo, Coahuila, Mexico;1. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea;2. Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea;3. Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;4. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;2. University of Chinese Academy of Sciences, Beijing 100039, China;1. Institute of Biomolecular Chemistry, National Council of Research (C.N.R.), Via Campi Flegrei 34, 80078, Pozzuoli, Naples, Italy;2. Department of Science and Technology, University of Naples “Parthenope”, Centro Direzionale, Isola C4, 80143, Naples, Italy;1. Department of Microbiology, Stellenbosch University, Private Bag X1, 7602 Matieland, Stellenbosch, South Africa;2. Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), Università di Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, Padova, Italy;1. Center of Innovative and Applied Bioprocessing, Sector 81, Mohali 160071, Punjab, India;2. Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Trivandrum 695019, Kerala, India;3. Bioenergy and Energy Planning Research Group, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland |
| |
| Abstract: | In this study, five recombinant Saccharomyces cerevisiae strains were compared for their xylose-fermenting ability. The most efficient xylose-to-ethanol fermentation was found by using the industrial strain MA-R4, in which the genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis along with an endogenous xylulokinase gene were expressed by chromosomal integration of the flocculent yeast strain IR-2. The MA-R4 strain rapidly converted xylose to ethanol with a low xylitol yield. Furthermore, the MA-R4 strain had the highest ethanol production when fermenting not only a mixture of glucose and xylose, but also mixed sugars in the detoxified hydrolysate of wood chips. These results collectively suggest that MA-R4 may be a suitable recombinant strain for further study into large-scale ethanol production from mixed sugars present in lignocellulosic hydrolysates. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
