Characterization of a metal resistant Pseudomonas sp. isolated from uranium mine for its potential in heavy metal (Ni2+, Co2+, Cu2+, and Cd2+) sequestration |
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| Authors: | Sangeeta Choudhary Pinaki Sar |
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| Affiliation: | 1. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China;2. Guangdong Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China;1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China;2. Molecular Microbiology, Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), 06120, Germany |
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| Abstract: | ![]()
Heavy metal sequestration by a multimetal resistant Pseudomonas strain isolated from a uranium mine was characterized for its potential application in metal bioremediation. 16S rRNA gene analysis revealed phylogenetic relatedness of this isolate to Pseudomonas fluorescens. Metal uptake by this bacterium was monophasic, fast saturating, concentration and pH dependent with maximum loading of 1048 nmol Ni2+ followed by 845 nmol Co2+, 828 nmol Cu2+ and 700 nmol Cd2+ mg?1 dry wt. Preferential metal deposition in cell envelope was confirmed by TEM and cell fractionation. FTIR spectroscopy and EDX analysis revealed a major role of carboxyl and phosphoryl groups along with a possible ion exchange mechanism in cation binding. Binary system demonstrated selective metal binding affinity in the order of Cu2+ > Ni2+ > Co2+ > Cd2+. A comparison with similar metal uptake reports considering live bacteria strongly indicated the superiority of this strain in metal sequestration, which could be useful for developing efficient metal removal system. |
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