Nuclease resistant methylphosphonate-DNA/LNA chimeric oligonucleotides |
| |
| Authors: | Koji Nagahama Rakesh N Veedu Jesper Wengel |
| |
| Institution: | Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M 5230, Denmark |
| |
| Abstract: | Synthesis of chimeric 9-mer oligonucleotides containing methylphosphonate-linkages and locked nucleic acid (LNA) monomers, their binding affinity towards complementary DNA and RNA, and their 3′-exonucleolytic stability are described. The obtained methylphosphonate-DNA/LNA chimeric oligonucleotides display similarly high RNA affinity and RNA selectivity as a corresponding 9-mer DNA/LNA chimeric oligonucleotide, but much higher resistance towards 3′-exonucleolytic degradation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
