Optimization of adsorption conditions of papain on dye affinity membrane using response surface methodology |
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| Authors: | Sai-Nan Su Hua-Li Nie Li-Min Zhu Tian-Xiang Chen |
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| Affiliation: | 1. Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA;2. Department of Environmental Sciences, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA;3. Finnish Environment Institute, Helsinki, Finland;1. College of Chemistry and Material Sciences, Hebei Normal University, Shijiazhuang 050024, PR China;2. College of Chemical Technology, Shijiazhuang University, Shijiazhuang 050035, PR China;1. Petroleum and Chemical Engineering, Universiti Teknologi Brunei, Brunei Darussalam;2. Department of Chemical Engineering, Faculty of Engineering, University of Ilam, Ilam, Iran;3. Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, 15875-4413, Iran;4. Department of Chemical and Biomolecular Engineering, National University of Singapore, Engineering Drive 4, Singapore, 117585, Singapore;5. University of Stuttgart, Institute of Chemical Technology, Faculty of Chemistry, D-70550, Stuttgart, Germany |
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| Abstract: | ![]()
The adsorption of papain on Reactive Blue 4 dye–ligand affinity membrane was investigated in a batch system. The combined effects of operating parameters such as initial pH, temperature, and initial papain concentration on the adsorption were analyzed using response surface methodology. The optimum adsorption conditions were determined as initial pH 7.05, temperature 39 °C, and initial papain concentration 11.0 mg/ml. At optimum conditions, the adsorption capacity of dye–ligand affinity membrane for papain was found to be 27.85 mg/g after 120 min adsorption. The papain was purified 34.6-fold in a single step determined by fast protein liquid chromatography. More than 85% of the adsorbed papain was desorbed using 1.0 M NaCl at pH 9.0 as the elution agent. The purification process showed that the dye–ligand immobilized composite membrane gave good separation of papain from aqueous solution. |
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